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Literature summary extracted from

  • Li, Y.; Zheng, Y.; Geng, Q.; Liu, F.; Zhang, Z.; Xu, J.; Yu, H.
    Secretory expression of cyclohexanone monooxygenase by methylotrophic yeast for efficient omeprazole sulfide bio-oxidation (2021), Bioresour. Bioprocess., 8, 81 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.14.13.22 synthesis extracellular production of engineered CHMO by Pichia pastoris. The recombinant CHMO shows a higher flavin occupation rate than that produced by Escherichia coli, accompanied by a 3.2fold increase in catalytic efficiency. At a cell density of 150 g/l cell dry weight, a recombinant CHMO production rate of 1,700 U/l is achieved. By directly employing the pH adjusted supernatant as a biocatalyst, 10 g/l of pyrmetazole is almost completely transformed into the corresponding (S)-sulfoxide, with > 99% enantiomeric excess Acinetobacter sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.22 0.00071
-
 NADPH recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication Acinetobacter sp.
1.14.13.22 0.0011
-
 NADPH recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication Acinetobacter sp.
1.14.13.22 0.026
-
 pyrmetazole recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication Acinetobacter sp.
1.14.13.22 0.028
-
 pyrmetazole recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication Acinetobacter sp.

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.22 Acinetobacter sp. P12015
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.22 pyrmetazole + NADPH + H+ + O2
-
 Acinetobacter sp. pyrmetazole (S)-sulfoxide + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.22 CHMO
-
 Acinetobacter sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.22 0.34
-
 NADPH recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication Acinetobacter sp.
1.14.13.22 0.35
-
 pyrmetazole recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication Acinetobacter sp.
1.14.13.22 0.82
-
 NADPH recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication Acinetobacter sp.
1.14.13.22 1.12
-
 pyrmetazole recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication Acinetobacter sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.14.13.22 12.5
-
 pyrmetazole recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication Acinetobacter sp.
1.14.13.22 43.4
-
 pyrmetazole recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication Acinetobacter sp.
1.14.13.22 304
-
 NADPH recombinant engineered enzyme expressed in Escherichia coli, pH 8.0, temperature not specified in the publication Acinetobacter sp.
1.14.13.22 1154
-
 NADPH recombinant engineered enzyme expressed in Pichia pastoris, pH 8.0, temperature not specified in the publication Acinetobacter sp.